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KMID : 0903519840270010040
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1984 Volume.27 No. 1 p.40 ~ p.44
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Gibberellin - like Activities Produced by mycorrhizal fungus , Pisolithus tinctorius
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Abstract
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Experiments on the GA production ability by ectomycorhizal fungus, Pisolithus tinctorius was carried out to investigate specific physiological phenomena of growth increase in host plants by formation of mycorrhizae, The culture extract of P. tinctorius was purified by solvent fractionation, sephadex LH-20 chromatography, silica gel partition chromatography and TLC, successively. GA activities in the purified GA fractions were monitored by micro-drop bioassay using dwarf rice seedlings, $quot;Tan-ginbozu$quot;. 30∼60% EtOAc election fractions of silica gel pardon chromatography and the zone of Rf 0.1∼0.4, 0.6∼0.8 of TLC exhibited the GA-like activities. The GA activities were increased with the more treated amount of culture extracts. This activity in 100㎖ of culture solution was equivalent to 0.1ng of GA₃.
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